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. 2019 Jan 24;104(9):1879–1891. doi: 10.3324/haematol.2018.208819

Figure 6.

Figure 6.

NOX1 and NOX2 are required for the induction of superoxide anion formation and platelets activation by oxidized low density lipoprotein (oxLDL). Oxygen radical generation (A) and platelet aggregation (B) in response to oxLDL were measured as described. 10 μM of scrambled NoxA1ds (scNoxA1ds), NoxA1ds, scrambled Nox2ds-tat (scNox2ds-tat), Nox2ds-tat or 100 unit/mL of PEG-SOD were pre-incubated 10 minutes (min) before platelet stimulation with 50 ng/mL oxLDL. Examples of electron paramagnetic resonance (EPR) traces and aggregation curves are representative of 4 independent experiments. Statistical analysis was performed by one-way ANOVA with Bonferroni post-hoc test. *P<0.05. N=3 for (B) and n=4 for (A). The effect of oxLDL as platelet modulator was investigated by traditional aggregometry (C and D). Pre-incubation with 50 ng/mL oxLDL for 10 min was followed by stimulation with 3 μg/mL collagen (C) or 0.03 unit/mL thrombin (D). In order to test the dependence of the modulatory effect of oxLDL on NOX1 and NOX2, 10 μM scrambled NoxA1ds (scNoxA1ds), NoxA1ds, scrambled Nox2ds-tat (scNox2ds-tat), Nox2ds-tat were pre-incubated (10 min before addition of oxLDL). Aggregation curves are representative of 4 independent experiments.