Figure 8.

Experiments on transgenic mice suggest that NOX1 and NOX2 are required for the effect of amyloid peptide β 1-42 and oxidized low density lipoprotein (oxLDL). Platelets from wild-type, NOX1^−/− or NOX2^−/−mice were stained with DiOC6 and the Bioflux platform (Fluxion, San Francisco, CA, USA) was utilized to assess the thrombus formation induced by collagen under physiological flow. (A) Ibidi Vena8⁺ flow chambers were coated with 0.05 mg/mL collagen and whole blood was treated with 50 ng/mL native LDL (nLDL) or oxLDL. (C) Ibidi Vena8+ flow chambers were coated with 20 μM Aβ1-42 or scrambled control peptide (ScAβ1-42). The shear rate utilized was 1,000 sec^-1, which leads to thrombus formation. Images were taken at 10 minutes (min) of flow and are representative of 4 independent experiments. Images were quantified by assessing the surface area coverage by platelets with Image J. Data are representative of 4 independent experiments. Statistical analysis was performed by one-way ANOVA with Bonferroni post-hoc test. *P<0.05. N=4 for (A and C). Aggregation experiments were performed by pre-treating platelets with 50 ng/mL native LDL (nLDL) or oxLDL (B) or 20 μM Aβ1-42 or scrambled control peptide (ScAβ1-42) (D) for 10 min. Low level aggregation was then stimulated with either 10 μg/mL collagen or 0.03 unit/mL thrombin, as indicated. Aggregation data are representative of 3 independent experiments.