Figure 1.

Tspan18 is highly expressed by endothelial cells. (A) Quantitative real-time polymerase chain reaction (qPCR) was carried out for Tspan18 using cDNA derived from a panel of mouse tissues. Data were normalized for the HPRT housekeeping gene and adjusted such that the lung value was 100 in each experiment. Error bars represent Standard Error of Mean from three independent tissue samples. (B) RNA-Seq data from major cell types in mouse lung, generated by Du et al.,⁴⁰ was used to show Tspan18 mRNA expression levels as fragments per kilobase of transcript sequence per million mapped fragments (FPKM). (C) RNA-Seq data from major cell types in mouse brain, generated by Zhang et al.,⁴¹ was used to show Tspan18 mRNA expression levels as described in (B). (D) qPCR was carried out for Tspan18 on cDNA derived from a panel of primary human cells [dermal fibroblasts, aortic smooth muscle, hepatocytes, peripheral blood leukocytes from buffy coat and human umbilical vein endothelial cells (HUVEC)], and human cell lines (HEK-293T human embryonic kidney cells, MDA-MB-231 epithelial cells, DAMI megakaryocytic cells, HEL and K562 erythroleukemia cells, U937 monocytic cells, Jurkat and HPB-ALL T cells, DG75 and Raji B cells and HMEC-1 microvascular endothelial cells). Data were normalized for actin and adjusted such that the HUVEC value was 100 in each experiment. Error bars represent Standard Error of Mean from two independent cell samples.