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. Author manuscript; available in PMC: 2019 Sep 1.
Published in final edited form as: Plant J. 2017 Oct 9;92(3):426–436. doi: 10.1111/tpj.13664

Figure 1. BIC1 antagonizes the CRY-mediated blue light inhibition of hypocotyl growth.

Figure 1.

(a) The representative hypocotyl image of the WT, rdr6, bic1bic2, cry1cry2, bic1bic2cry1cry2, Myc-CRY2, Flag–BIC1, and Myc-CRY2/Flag–BIC1 grown in blue light (10 μmol m−2 sec−1) for 5 days. Scale bar = 1 cm. All transgenic lines, including Myc-CRY2, Flag–BIC1, and Myc-CRY2/Flag–BIC1 are in the rdr6 mutant that has no defects in photo-responses to avoid post-transcriptional gene silencing.

(b) Growth kinetics analysis of indicated genotypes. Seedlings germinated and grown in continuous blue light (10 μmol m−2 sec−1) were imaged every 15 min, lengths of hypocotyl of three seedlings were averaged and the error bar shows the standard deviation. Three types of growth kinetics are grouped and labeled as I, II and III.

(c) Immunoblots of protein samples prepared from 6-day-old seedlings of indicated genotypes grown in continuous blue light (10 μmol m−2 sec−1) were probed with anti-CRY2, anti-Flag or anti-HSP90 antibody, respectively. The endogenous CRY2 (CRY2) is resistant to degradation in plants overexpressing BIC1.