Figure 2.

Altered NLPR3 expression modulated the proliferation, clonogenicity, migration, and invasion in endometrial cancer cells. Ishikawa and HEC-1A cells were transduced with lentivirus for NLPR3 silencing. (A) Western blot analysis of relative NLRP3 levels in NLRP3 silence cells. The proliferation (B), clonogenicity (C), invasion (D), and wound healing (E) in different groups of cells were determined. Ishikawa and HEC-1A cells were transduced with lentivirus for NLPR3 overexpression. (F) Western blot analysis of relative NLRP3 levels in NLRP3 overexpressing cells. The relative levels of NLPR3, IL-1β, and caspase-1 were determined by Western blot. Data are representative images or expressed as mean ± SEM of each group from three separate experiments. The proliferation (F), migration (mig, G), and invasion (inva, H) in different groups of cells were determined. (I) Caspase-1 inhibitor YVAD-cmk inhibits the proliferation of endometrial cells. *P<0.05, **P<0.01, ***P<0.001.

Abbreviations: ISK, Ishikawa; Casp-1, Caspase-1.