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. 2019 Sep 2;9:11607. doi: 10.1038/s41598-019-48104-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of the activity of pHLIP- siCEACAM6 in A549 cells. (a) Western blotting analysis of CEACAM6 protein levels in A549 cells incubated with pHLIP-siCEACAM6 at pH 6.2. (b) Flow cytometric analysis of A549 cells incubated with pHLIP-siCEACAM6 at neutral and acidic pH values. (c) Effects of CEACAM6 inhibition on A549 cell viability at neutral and acidic pH values. Data are shown as mean ± s.d.; ***P < 0.001.