Increased longevity of the eat-2(ad465) strain is suppressed by atgl-1(P87S). (A) N2 and atgl-1(P87S) worms were split into Fed and Fasted groups and stained with Oil Red O. (B) Triglyceride content was measured in Fed and Fasted groups of N2 and atgl-1(P87S) worms. (C) N2 and eat-2(ad465) worms were stained with Oil Red O. (D) Triglyceride content was measured in N2 and eat-2(ad465) worms. (E) RNA was extracted from N2 and eat-2(ad465) worms, and atgl-1 mRNA levels were measured by qRT-PCR; actin-1/3 was used for normalization. (F) The lifespan of eat-2(ad465) worms is extended by 35.3% in comparison to N2 (p value 1.0e-3, log-rank with Boneferroni correction), while the lifespan of eat-2(ad465); atgl-1(P97S) worms is decreased by 33.1% in comparison to eat-2(ad465) (p value < 1.0e-10, log-rank with Bonferroni correction). Panels B, D, and E show an average result of three independent experiments ±SEM, p values were calculated by unpaired two-tailed t-test. *p< 0.05; **p < 0.01; ***p < 0.001.