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. 2019 Jul 5;27:75–82. doi: 10.1016/j.molmet.2019.07.001

Figure 3.

Figure 3

Expression of ATGL-1 is regulated by the daf-2/daf-16 pathway, and atgl-1(P87S) suppresses longevity of the daf-2 mutant. (A) L4 stage worms were visualized by fluorescence microscopy (200X, equal exposure times). Bar – 50 μM. (B) Quantification of the results shown in panel A by ImageJ (10 randomly selected worms per group). (C) Expression of the atgl-1 mRNA was measured by RT-qPCR; actin-1/3 was used for normalization. (D) The lifespan of daf-2(e1370) worms is increased by 131.1% in comparison to N2 (p value < 1.0e-10, log-rank with Boneferroni correction), while the lifespan of daf-2(e1370); atgl-1(P97S) worms is decreased by 46.3% in comparison to daf-2(e1370) (p value < 1.0e-10, log-rank with Boneferroni correction). Panel D shows an average result of three independent experiments ±SEM, p values were calculated by unpaired two-tailed t-test. **p < 0.01; ***p < 0.001, n.s. – not significant.