Figure 5.

Surface temperature assays of skins from mice with deficient skin-associated lipid barriers. FLIR imaging of skins on dry and wet blocks and quantitation of each assay is shown: When [T0-T1] is positive, skin surface is cooler than block, reflecting degree of insulation. On wet blocks, when [T0-T3] is positive, skin surface is cooler than block, as a result of rate of evaporative cooling combined with insulation properties. Note the background in the pictures is [T2], the saturated water pad, always the coolest part of the picture, showing maximum rates of evaporative cooling from the warm block (measured as a TEWL rate of >300 g/m²/h). A. DGAT1 KO; B. SKO; C. Sdc1KO; D. obese A^y mice; n ≥ 3 mice per assay. Data are expressed as mean ± SEM. Statistical analysis was performed with unpaired 2-tailed t test (****P < 0.0001; *P < 0.05; ns = not significant).