Fig. 3. Closed channel blocking effect of mosapride on the 5-hydroxytryptamine type 3 (5-HT₃) receptor.

(A) Representative trace of 5-HT₃ receptor currents induced by 5-HT (10 µM) alone, co-application of mosapride (10 µM) and 5-HT, and co-application 1 min after mosapride pretreatment. The open horizontal bar indicates the 5-HT application period and the closed bar indicates mosapride application. (B) Averaged inhibitory effect on the peak amplitude of 5-HT₃ receptor currents. Pretreatment for 1 min significantly increased the inhibitory effect of mosapride (n = 10, ^*p < 0.001, paired t-test). The data are expressed as means ± standard error of the mean.