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. 2019 Aug 28;12:7015–7024. doi: 10.2147/OTT.S213583

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© 2019 Wei et al.

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miR-30a-3p targets DNMT3a. (A) The mRNA levels of miR-30a-3p in A549 cells measured by RT-qPCR 48 hrs after transfection with miR-30a-3p. (B, C) Overexpression of miR-30a-3p inhibited the protein expression levels of DNMT3a in A549 cells. Data are reported as mean ± SD (n=3). **P<0.1 versus NC group. (D) Luciferase assay miR-30a-3p binding sites in the WT-3ʹUTR and Mut-3ʹUTR of DNMT3A were cloned into pmirGLO Dual-Luciferase miRNA Target Expression vector. The normalized firefly luciferase activity was obtained by firefly luciferase activity/Renilla luciferase activity. Data are reported as mean ± SD (n=3). **P<0.1 versus Con group.

Abbreviations: Con, control group; NC, cells transfected with miR-control vector group; miR-30a-3p, cells transfected miR-30a-3p vector group.