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. 2019 Aug 28;12:7015–7024. doi: 10.2147/OTT.S213583

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© 2019 Wei et al.

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Silencing DNMT3a suppresses lung cancer cell proliferation and induces G1-phase arrest and cell apoptosis in A549 cells, and upregulating DNMT3a induces lung cancer cell proliferation and cycle progression and inhibits cell apoptosis in overexpression miR-30a-3p A549 cells. (A, B) Analysis of protein expression of DNMT3a in A549 cells at 48 hrs after transfection. (C) The effect of DNMT3a knockdown or overexpression on the proliferation of A549 cells were evaluated by MTT assay at 24, 48 and 72 hrs. (D, E) The cell cycle was determined in A549 cells at 48 hrs after DNMT3a knockdown or overexpression. (F, G) Cell apoptosis was detected in A549 cells at 48 hrs after DNMT3a knockdown or overexpression. (H, I) Analysis of protein expression associated with cell cycle and apoptosis in A549 cells at 48 hrs after transfection. Data are reported as mean ± SD (n=3). **P<0.1 versus si-con group. ^##P<0.01 versus EV + miR-30a-3p group.

Abbreviations: Si-con, cells transfected with DNMT3a empty vector group; siDMNT3a, cells transfected with DNMT3a interference vector; EV+miR-30a-3p, cells transfected with miR-30a-3p overexpression vector and DNMT3a empty vector group; miR-30a-3p+DNMT3a, cells transfected miR-30a-3p and DNMT3a overexpression vector group.