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. 2019 Aug 28;12:6991–7004. doi: 10.2147/OTT.S214881

Figure 6.

Figure 6

Reintroduction of KPNA2 prevents the tumor-suppressive effects of miR-411 upregulation on Eca109 and TE-1 cells. (A) MiR-411–overexpressing Eca109 and TE-1 cells were next transfected with pc-KPNA2 or pcDNA3.1. At 48 h after transfection, the cells were collected and subjected to Western blotting for the determination of KPNA2 protein expression. *P<0.05 vs miR-NC. #P<0.05 vs miR-411 mimics+pcDNA3.1. (B–E) The proliferation, apoptosis, migration, and invasiveness of Eca109 and TE-1 cells treated as described above were evaluated by the CCK-8 assay, flow cytometry, and Transwell migration and invasion assays, respectively. *P<0.05 vs miR-NC. #P<0.05 vs miR-411 mimics+pcDNA3.1.