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. 2019 Jul 29;38(17):e100772. doi: 10.15252/embj.2018100772

Figure EV2. Bacterial two hybrid (BTH) assays showing weak interactions between Bd1971 and c‐di‐GMP synthesizing proteins.

Figure EV2

  1. Spot tests of co‐transformants of either Bd0742 (DgcB) or Bd1434 (DgcC) with Bd1971. Positive control (+) was co‐transformants with pUT18c‐zip and pKT25‐zip, negative control (−) was the empty vectors. The co‐transformants were plated on LB‐X‐Gal medium with positive interaction resulting in blue coloration. Interactions with Bd1971 in pUT18c were always positive (examples on right of diagram), however with Bd1971 in pUT25, the results were inconsistent (three examples shown 1, 2, 3 on left of diagram), indicative of a weak or transient interaction. Images are representative of at least 3 independent experiments.
  2. Confirmation of BTH interactions by β‐Galactosidase activity assays. The interaction between Bd1971 in pUT25 was significant with diguanylate cyclase Bd0742, but not with Bd1434. *P > 0.05 by Student's t‐test. Data are from three independent experiments.

Source data are available online for this figure.