Figure 3.

The effect of PSD knockdown on transcriptional markers of regeneration, atrophy, denervation, and inflammation, DNA and protein synthesis as well as free amino acid levels. AAV vectors were injected in the tibialis anterior (TA) of 8 week old mice. Studies to assess gene expression, DNA and protein synthesis and free amino acid levels were conducted 8 weeks after AAV administration (i.e. at 16 weeks of age). A: The effect of PSD knockdown on the expression of atrophy, denervation and inflammation-related genes (N = 12; 6 female, 6 male). B: Plasma ²H₂O enrichment after 1 week (i.e. between weeks 7 and 8 after AAV administration) of heavy water labeling (N = 14; 8 female, 6 male). C: DNA synthesis in TA muscles over the 1 week heavy water labeling period (N = 6, 3 female, 3 male). D: Concentration of protein-bound alanine in TA muscle from the 1 week heavy water labeling study (N = 14; 8 female, 6 male). E: Fraction of newly synthesized protein in TA muscle from the 1 week heavy water labeling study (N = 14; 8 female, 6 male). F; The absolute amount of newly synthesized protein in the TA muscle from the 1 week heavy water labeling study (N = 14; 8 female, 6 male). G: Plasma ²H₂O enrichment over the 4 week i.e. between weeks 4 and 8 after AAV administration) heavy water labeling study (N = 21; 13 female, 8 male). H: DNA synthesis in TA muscles over the 4 week heavy water labeling period (N = 21; 13 female, 8 male). I: Concentration of protein-bound alanine in TA muscle from the 4 week heavy water labeling study (N = 21; 13 female, 8 male). J: Fraction of newly synthesized protein in the TA muscle from the 4 week heavy water labeling study (N = 21; 13 female, 8 male). K: The absolute amount of newly synthesized protein in the TA muscle from the 4 week heavy water labeling study (N = 21; 13 female, 8 male). L: Concentrations of free amino acids in TA muscle (N = 7; 3 female, 4 male). Data are mean ± SEM. Data were analyzed using paired t-tests. ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001. ND, not detected.