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. 2019 Jun 27;27:33–46. doi: 10.1016/j.molmet.2019.06.020

Figure 7.

Figure 7

Effect of short-term (4 week) disruption of the PSD pathway on mitochondrial structure and function. AAV vectors were injected in the tibialis anterior (TA) of 8 week old mice. Studies to assess aspects of mitochondrial structure and function were conducted 4 weeks after AAV administration (i.e. at 12 weeks of age). A: Succinate dehydrogenase staining. Scale bar is 200 μm for the panel (whole mount) and 50 μm for the right panel. B: Representative electron micrograph of TA muscle. Scale bar is 2 μm for the left electron micrograph, 500 nm for the middle and 200 nm for the right. C: Representative western blot of parkin protein levels. D: Representative western blot of OPA1 protein levels. E: Quantification of parkin and OPA1 protein content (N = 7; 4 female, 3 male). F: Cardiolipin content in TA muscles (N = 7; 4 female, 3 male). G: Representative western blots of the OXPHOS proteins in Complex I–V of the electron transport chain. H: Quantification of the OXPHOS proteins (N = 7; 4 female, 3 male). I: Citrate synthase and β-HAD activity in TA muscle (N = 6 male). J: Basal and maximal oxygen consumption rates in TA muscles (N = 7; 2 female, 5 male). K: Concentration of TCA cycle metabolites (N = 11; 5 female, 6 male). Data are mean ± SEM. Data were analyzed using paired t-tests.∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001.