Fig. 5.

LRP in LBD SNpc and cingulate cortex is similarly detected across a panel of αsyn antibodies. a Representative sections of the SNpc from an LBD case labeled with 5 different αsyn antibodies as indicted in the top, left corners without or with FA as indicated. Insets display LBs in pigmented neurons. Within the SNpc, abundance of pathology is similar regardless of antibody used or antigen retrieval albeit with minor differences. Scale bar 50 μm. b Representative staining of the cingulate cortex from an LBD case labeled with 5 different αsyn antibodies without or with FA as indicated. Insets display cortical LBs. Within the cingulate, pathology is similar for most antibody and antigen retrieval conditions. αsyn-positive neurites are slightly more apparent with FA retrieval or C-terminal antibodies, particularly 94-3A10 with FA which is reflected in the quantitative positivity analysis. Scale bar 50 μm. c Sections from the cingulate of an AD/ALB case with no LRP were stained with 4 αsyn antibodies without or with FA, as indicated. Little to no positive staining was detected in the AD/ALB cingulate cortex with any of these antibodies. Scale bar 50 μm