Figure 2.

AtMIOX4 OE line accumulates high ascorbate and enhanced growth. (a) The AtMIOX4:pBIB‐Kan construct containing the myo‐inositol oxygenase (AtMIOX4) and the neomycin phosphotransferase II (nptII) ORFs. 35S2E‐P: cauliflower mosaic virus double‐enhanced promoter, TEV, tobacco etch virus translational enhancer; NOS‐P, promoter, NOS‐T terminator. (b) Total foliar AsA content of A. thaliana MIOX4 plants. Values are means ± SEM (n = 6), *p < .05 at 0.05 significance level. (c) Southern blot, illustrating the number of copies of the transgene in multiple AtMIOX4 lines. DNA was digested with either KpnI or SacI; WT: wild type (negative control); arrows indicate molecular weight marker. (d) The projected leaf surface area of AtMIOX4 L21 line is significantly higher compared to WT control. Data are means ± SEM (n = 8), p < .001 at 0.05 significance level