Skip to main content
. 2019 Sep 2;3(9):e00165. doi: 10.1002/pld3.165

Figure 6.

Figure 6

Increased auxin metabolism in high ascorbate line. (a) Transcripts related to auxin biosynthesis, auxin–amino acid conjugate hydrolases, auxin transport, and auxin response small RNA were up‐regulated in the MIOX4 transcriptome compared to WT. (b) Expression of transcripts related to auxin metabolism was validated using RT‐qPCR. Data are means ± SEM (n = 3). (c) Active auxin and inactive auxin (IAA‐Asp) were significantly higher in MIOX4 OE compared to WT as shown by LC‐MS/MS. Data are means ± SEM (n = 5). (d,e) Hypocotyl length of seedlings grown in dark conditions and primary root length of seedlings grown in light conditions were significantly longer in MIOX4 compared to WT 9 days after germination. Data are means ± SEM (n = 20). (f,g) Hypocotyl epidermal cell length was significantly higher in MIOX4 OE compared to WT. Data are means ± SEM (n = 30). **Indicates p < .01, ***indicates p < .001, and ****indicates p < .0001 at 0.05 significance level