Figure 10.
Antioxidant crosstalk in MIOX4 OE. (a) The ascorbate recycling enzyme DHAR1 and glutathione reductase (GRX480) were significantly up‐regulated, whereas ascorbate peroxidase 5 (APX5) was significantly down‐regulated in the MIOX4 OE transcriptome compared to WT. (b) RT‐qPCR validation of up‐regulated and down‐regulated transcripts involved in ascorbate–antioxidant crosstalk. Data are means ± SEM (n = 3). (c) H2O2 accumulation is higher in foliar tissue of MIOX4 OE line compared to WT. Data are means ± SEM (n = 4). *Indicates p < .05, **indicates p < .01, and ***indicates p < .001 at 0.05 significance level. APX, Ascorbate peroxidase; AsA, ascorbate; GR, glutathione reductase; GSH, reduced glutathione; GSSG, oxidized glutathione; H2O2, hydrogen peroxide