Figure 11.

Biotic stress response transcripts are differentially expressed in high ascorbate line. (a) Transcripts related to defense hormone biosynthesis, biotic stress response transcription factors, and specialized metabolite biosynthesis were up‐regulated while some transcripts related to cell wall metabolism were down‐regulated in MIOX4 OE compared to WT. (b) The up‐regulation of transcripts involved in biotic stress response was validated using RT‐qPCR. Data are means ± SEM (n = 3). (c) Defense hormone jasmonates (JA) and (d) jasmonic acid‐Ile (JA‐Ile) were significantly higher in MIOX4 OE line compared to WT. Data are means ± SEM (n = 5), *indicates p < .05, **indicates p < .01, and ***indicates p < .001 at 0.05 significance level