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. Author manuscript; available in PMC: 2019 Sep 2.
Published in final edited form as: J Neurochem. 2012 Aug 14;123(1):147–158. doi: 10.1111/j.1471-4159.2012.07859.x

Fig. 1.

Fig. 1

Schematic representation and expression of recombinant InsP3R1 constructs in primary neurons. (a) Protein domain structure of wt-InsP3R1 (top), calpain-cleaved InsP3R1 recombinant (middle), and calpain-cleaved InsP3R1 with the D2550A point mutation (bottom; arrowhead). Residues numbered according to rat type 1 SI+, SII+, SIII-sequence (protein accession NP_001007236.1). (b) Western blot analysis of whole-cell lysates from untransduced rat primary cortical cultures and primary cortical cultures transduced with AAV 2/1 expressing lacZ, capn-InsP3R1 D2550A, or capn-InsP3R1 at 1 week post-transduction (14 DIV). Carboxyl-terminal InsP3R1 antibody was used to detect endogenous and recombinant truncated rat InsP3R1. Antibody against actin was used as a loading control.