Skip to main content
. 2019 Aug 26;10:929. doi: 10.3389/fphar.2019.00929

Figure 2.

Figure 2

Cardiac remodeling induced by 10 days of Iso exposure in rats in vivo. (A) The gross morphology of the whole hearts. (B) Representative echocardiographic images from each of the corresponding hearts shown in (A). (C) HE staining of transverse LV sections (250×). Scale bars = 50 µm. (D) Masson’s trichrome staining showing collagen deposition in rat LV (100 ×). Cardiomyocytes and collagen fibers were stained as red and blue, respectively. Scale bars = 100 µm. (E) Statistical results of the cross sectional area of myofiber in different groups. N = 50 in each group. (F) Statistical results of fibrotic area which was expressed as the percentage of total area in each field, n = 5 in each group. (G) The heart/LV mass index. (H) The expression levels of Kir2.1 channel protein. Iso, isoproterenol; Zac, zacopride; Chlo, chloroquine; RS23597, RS23597-190; m-CPBG, m-chlorophenylbiguanide. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01 compared with control. ## P < 0.01 compared with Iso+Zac.