Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 25;42(4):1380–1390. doi: 10.3892/or.2019.7250

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Yue et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 3. — CLIC1 is essential for liver cancer cell migration and invasion, but not proliferation. (A) CLIC1 protein is overexpressed in liver cancer cells compared with normal liver epithelial cells. (B) Analysis of the mRNA expression level of CLIC1 by reverse transcription-quantitative PCR. *P<0.05, ***P<0.001 vs. HL-7702. (C) CLIC1 protein and (D) mRNA were reduced by siR-CLIC1 in liver cancer cells. (E) WST1 showed that CLIC1 had no effect on cell viability and cell growth. siR, small interfering RNA; CLIC1, chloride intracellular protein 1; NC, negative control; n.s., not significant. (F) Colony formation assays showed that CLIC1 had no effect on cell viability and cell growth. The cell migration of liver cancer cells after knockdown of CLIC1 was evaluated by (G) scratch wound-healing motility assay and (H) Transwell assay without Matrigel coating. Scale bar, 200 µm. (I) The cell invasion ability of liver cancer cells was evaluated by Matrigel-coated Transwell assay. Scale bar, 200 µm. ***P<0.001 vs. respective siR-NC. siR, small interfering RNA; CLIC1, chloride intracellular protein 1; NC, negative control; n.s., not significant.

Figure 3. — CLIC1 is essential for liver cancer cell migration and invasion, but not proliferation. (A) CLIC1 protein is overexpressed in liver cancer cells compared with normal liver epithelial cells. (B) Analysis of the mRNA expression level of CLIC1 by reverse transcription-quantitative PCR. *P<0.05, ***P<0.001 vs. HL-7702. (C) CLIC1 protein and (D) mRNA were reduced by siR-CLIC1 in liver cancer cells. (E) WST1 showed that CLIC1 had no effect on cell viability and cell growth. siR, small interfering RNA; CLIC1, chloride intracellular protein 1; NC, negative control; n.s., not significant. (F) Colony formation assays showed that CLIC1 had no effect on cell viability and cell growth. The cell migration of liver cancer cells after knockdown of CLIC1 was evaluated by (G) scratch wound-healing motility assay and (H) Transwell assay without Matrigel coating. Scale bar, 200 µm. (I) The cell invasion ability of liver cancer cells was evaluated by Matrigel-coated Transwell assay. Scale bar, 200 µm. ***P<0.001 vs. respective siR-NC. siR, small interfering RNA; CLIC1, chloride intracellular protein 1; NC, negative control; n.s., not significant.