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. 2019 Jul 25;42(4):1307–1318. doi: 10.3892/or.2019.7248

Figure 5.

Figure 5.

Inhibition of HIF-1α blocks hypoxia-induced upregulation of FoxM1. (A) PC3 (left panel) and DU145 (right panel) cells were cultured under normoxic or hypoxic conditions for different durations (0, 6, 12 and 24 h). HIF-1α and FoxM1 protein levels were detected by western blotting. (B) PC3 and DU145 cells were cultured under normoxic or hypoxic conditions for different durations (0, 6 12 and 24 h). HIF-1α and FoxM1 mRNA levels were detected by RT-qPCR. (C and D) PC3 and DU145 cells were transfected with specific RNAi duplexes targeting for HIF-1α or pretreated with HIF-1α inhibitor YC-1 for 24 h, and then exposed to hypoxia for an additional 24 h. Then, the protein expression levels of HIF-1α and FoxM1 were determined by western blotting. (E and F) Under similar conditions, the mRNA expression levels of FoxM1 were determined by RT-qPCR. Data are presented as the mean ± standard deviation of three replicates. *P<0.05. ctrl, control; FoxM1, Forkhead box M1; HIF-1α, hypoxia-inducible factor 1α; RNAi, RNA interference; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.