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. 2019 Aug 28;75(Pt 9):608–615. doi: 10.1107/S2053230X19011154

Table 2. Crystallization.

Method Vapor diffusion, hanging drop
Plate type EasyXtal 15-well plate, Qiagen
Temperature (K) 292
Protein concentration (mg ml−1) 6.2
Composition of reservoir solution 0.1 M MES pH 6.0, 20%(v/v) PEG Smear Low (polyethylene glycol 400, polyethylene glycol methyl ether 550, polyethylene glycol 600, polyethylene glycol 1000)
Additive solution 0.1 M spermidine
Volume and composition of drop 5 µl; 2.5 µl protein + 2.0 µl reservoir + 0.5 µl additive
Volume of reservoir (ml) 0.5