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. Author manuscript; available in PMC: 2019 Sep 2.
Published in final edited form as: Bioorg Med Chem. 2018 Jun 12;26(14):3899–3908. doi: 10.1016/j.bmc.2018.06.010

Fig. 2.

Fig. 2.

Compound 7g induces SW620 and HT29 cell cycle arrest at the G2/M checkpoint. (A) Cell cycle distribution was detected using flow cytometry in SW620 and HT29 cells. Both lines were treated with compound 7g for 48 h at different concentrations (0, 25, 50, and 75 μM). Cells were collected and treated with PI and RNase. (B) Quantification of SW620 and HT29 cells at various phases of the cell cycle. Each point represents the average of three independent experiments. (C) Western blotting was used to analyze the effect of compound 7g on expression of cell cycle-related proteins. SW620 and HT29 cells were treated with compound 7g for 48 h at different concentrations (0, 25, 50, and 75 μM) and were harvested, and whole protein lysates were extracted. The expression of cell cycle-related proteins (p53, p21, cyclin B1 and CDK1) was analyzed by western blot. Tubulin was used as a loading control. The relative density of cell cycle related-proteins was analyzed by the Tanon Gis Software. Data is shown as the mean ± SD. * = p < 0.05; ** = p < 0.01.