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. Author manuscript; available in PMC: 2019 Sep 2.
Published in final edited form as: Bioorg Med Chem. 2018 Jun 12;26(14):3899–3908. doi: 10.1016/j.bmc.2018.06.010

Fig. 4.

Fig. 4.

Effect of compound 7g on the PI3K/AKT/mTOR signaling pathway. (A) SW620 and HT29 cells were treated with compound 7g for 48 h at different concentrations (0, 25, 50, and 75 μM). Cells were collected and harvested for protein lysates. The expression levels of PI3K/AKT/mTOR signaling pathway related proteins (PTEN, p-AKT, AKT, p-mTOR, mTOR, 4E-BP1, p-4E-BP1) were analyzed by western blot. Tubulin was used as a loading control. (B) The relative density of PI3K/AKT/mTOR signing pathway related-proteins was analyzed by the Tannon Gis Software. Each data point is the average of three independent experiments. Data were shown as the mean ± SD. * = p < 0.05; ** = p < 0.1.