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. 2019 Aug 29;12:231–239. doi: 10.2147/JIR.S224834

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© 2019 Nguyen et al.

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EGCG suppressed neutrophil migration speed and traveled distance. The larvae at 4 dpf larvae were tail-resected and mounted in EGCG infused agar for 2 hr imaging. From the time-lapse records, neutrophils were detected by fluorescence microscopy. Motion parameters including velocity and distance were extracted using Fiji Manual Tracking. More dynamic tracks were recorded in the untreated control fish within the ROI, compared to the ECGC treated fish, in terms of the number of active tracks, distance, and speed. Quantitative analysis of event speed (LEFT) and distance (RIGHT) in ROI of zebrafish treated with vehicle and EGCG (300 and 600 μM) are plotted (mean ± SD, n=6–10, t-test).

Abbreviations: EGCG, epigallocatechin-3-gallate; ROI, region of interest.