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. 2019 Jun 21;198(1):57–70. doi: 10.1111/cei.13332

Figure 1.

Figure 1

Delineation of peripheral blood immune cells. For all staining panels, cells were first gated upon forward‐scatter (FSC)‐height versus FSC‐area for doublet discrimination, followed by gating on side‐scatter (SSC)‐area versus CD45 for distinguishing peripheral blood mononuclear cells (PBMCs). (a) Gating strategy for dendritic cell (DC), natural killer (NK) cell and monocyte panel; (b) gating strategy for B cell panel; (c) gating strategy for regulatory T cell (Treg) panel; and (d) gating strategy for T cell panel.