. 2019 Jun 21;198(1):57–70. doi: 10.1111/cei.13332

Table 1.

Marker combinations used for identification of cell subtypes in T cell, regulatory T cell (T_reg), B cell, dendritic cell (DC), natural killer (NK) cell and monocyte compartments

T cell panel		T_reg panel
CD3⁺ T cells	CD3⁺	CD4⁺ T regulatory cells (T_reg)	CD3⁺CD4⁺CD25⁺CD127^–
CD4⁺ T helper (Th) cells	CD3⁺CD4⁺	Memory T_reg	CD3⁺CD4⁺CD25⁺CD127^–CD45R0⁺CD194⁺
CD4⁺ naive Th cells	CD3⁺CD4⁺CD45RA⁺CD197⁺	HLA^–DR⁺ T_reg	CD3⁺CD4⁺CD25⁺CD127^–CD45R0⁺CD194⁺HLA^–DR⁺
CD4⁺ TEMRA	CD3⁺CD4⁺CD45RA⁺CD197^–	NK cell, DC and monocyte panel
CD4⁺ effector memory (EM) Th cells	CD3⁺CD4⁺CD45RA^–CD197^–	NK cells	CD3^–CD19^–CD20^–CD14^–CD56⁺
EM Th1	CD3⁺CD4⁺CD45RA^–CD197^–CD183⁺CD196^–	CD56⁺⁺CD16^– (CD56^brightCD16^–) regulatory NK cells	CD3^–CD19^–CD20^–CD14^–CD56⁺⁺CD16^–
EM Th2	CD3⁺CD4⁺CD45RA^–CD197^–CD183^–CD196^–	CD56⁺⁺CD16⁺ (CD5^brightCD16⁺) intermediate NK cells	CD3^–CD19^–CD20^–CD14^–CD56⁺⁺CD16⁺
EM Th17	CD3⁺CD4⁺CD45RA^–CD197^–CD183^–CD196⁺	CD56⁺CD16^– (CD56^dimCD16^–) NK	CD3^–CD19^–CD20^–CD14^–CD56⁺CD16^–
CD4⁺ central memory (CM) Th cells	CD3⁺CD4⁺CD45RA^–CD197⁺	CD56⁺CD16⁺ (CD56^dimCD16⁺) effector NK cells	CD3^–CD19^–CD20^–CD14^–CD56⁺CD16⁺
CM Th1	CD3⁺CD4⁺CD45RA^–CD197⁺CD183⁺CD196^–	Dendritic cells (DC)	CD3^–CD19^–CD20^–CD14^–CD56^–HLA^–DR⁺
CM Th2	CD3⁺CD4⁺CD45RA^–CD197⁺CD183^–CD196^–	Plasmacytoid DC	CD3^–CD19^–CD20^–CD14^–CD56^–HLA^–DR⁺CD123⁺CD11c^–
CM Th17	CD3⁺CD4⁺CD45RA^–CD197⁺CD183^–CD196⁺	Myeloid DC (mDC)	CD3^–CD19^–CD20^–CD14^–CD56^–HLA^–DR⁺CD123^–CD11c⁺
I activated CD4⁺ T cells	CD4⁺HLA^–DR⁺CD38^–	slan^– CD16^– mDC	CD3^–CD19^–CD20^–CD14^–CD56^–HLA^–DR⁺CD123^–CD11c⁺slan^–CD16^–
II activated CD4⁺ T cells	CD4⁺HLA^–DR⁺CD38⁺	slan^– CD16⁺ mDC	CD3^–CD19^–CD20^–CD14^–CD56^–HLA^–DR⁺CD123^–CD11c⁺slan^–CD16⁺
Non‐activated CD4⁺ T cells	CD4⁺HLA^–DR^–CD38^–	slan⁺ CD16⁺ mDC	CD3^–CD19^–CD20^–CD14^–CD56^–HLA^–DR⁺CD123^–CD11c⁺slan⁺CD16⁺
III activated CD4⁺ T cells	CD4⁺HLA^–DR^–CD38⁺	Monocytes	CD3^–CD19^–CD20^–CD14⁺
CD8⁺ cytotoxic lymphocyte (CTL) T cells	CD3⁺CD8⁺	CD14⁺CD16^– classical monocytes	CD3^–CD19^–CD20^–CD14⁺CD16^–
CD8⁺ naive CTL	CD3⁺CD8⁺CD45RA⁺CD197⁺	CD14⁺CD16⁺ non^–classical monocytes	CD3^–CD19^–CD20^–CD14⁺CD16⁺
CD8⁺ TEMRA CTL	CD3⁺CD8⁺CD45RA⁺CD197^–	B cell panel
CD8⁺ EM CTL	CD3⁺CD8⁺CD45RA^–CD197^–	CD19⁺ B cells	CD3^–CD19⁺
CD8⁺ CM CTL	CD3⁺CD8⁺CD45RA^–CD197⁺	Switched memory B cells (SM B cell)	CD3^–CD19⁺IgD^–CD27⁺
I activated CD8⁺ T cells	CD8⁺HLA^–DR⁺CD38^–	Plasmablasts	CD3^–CD19⁺IgD^–CD27⁺CD38⁺CD20^–
II activated CD8⁺ T cells	CD8⁺HLA^–DR⁺CD38⁺	Preswitch memory B cells (PSM B cell)	CD3^–CD19⁺IgD⁺CD27⁺
Non‐activated CD8⁺ T cells	CD8⁺HLA^–DR^–CD38^–	Exhausted memory B cells	CD3^–CD19⁺IgD^–CD27^–
III activated CD8⁺ T cells	CD8⁺HLA^–DR^–CD38⁺	Naive B cells	CD3^–CD19⁺IgD⁺CD27^–
CD4⁺CD8⁺ double‐positive T cells	CD3⁺CD4⁺CD8⁺	Transitional B cells	CD3^–CD19⁺IgD⁺CD27^–CD38⁺CD24⁺
CD4^–CD8^– double‐negative T cells	CD3⁺CD4^–CD8^–

TEMRA = T cell effector memory subset that expresses CD45RA; HLA‐DR = human leukocyte antigen DR, slan = 6‐sulfo LacNac.