Table 3.
List of fluorochromes, cell surface markers and respective clones of monoclonal antibodies used for the identification of cell subtypes in T cell, regulatory T cell (Treg), B cell, dendritic cell (DC), natural killer (NK) cell and monocyte compartments
| Fluorochromes | T cell panel | Treg panel | B cell panel | DC/NK cell/monocyte panel | ||||
|---|---|---|---|---|---|---|---|---|
| Marker | Clone | Marker | Clone | Marker | Clone | Marker | Clone | |
| FITC | CD24* | ML5 | Slan**** | DD‐1 | ||||
| AF488 | CD183 (CXCR3)** | G025H7 | ||||||
| PerCP‐Cy5.5 | CD4* | RPA‐T4 | CD4* | RPA‐T4 | CD19* | HIB19 | CD123* | 7G3 |
| PE | CD197 (CCR7)* | 150503 | CD25* | M‐A251 | CD56*** | MY31 | ||
| PE‐Cy7 | CD45RA* | HI100 | CD194 (CCR4)* | 1G1 | IgD** | IA6‐2 | CD11c* | B‐LY6 |
| APC | CD38* | HIT2 | CD38* | HIT2 | CD16* | B73.1 | ||
| AF647 | CD127* | hIL‐7R‐M21 | ||||||
| AF700 | CD45** | HI30 | CD45** | HI30 | CD45** | HI30 | CD45** | HI30 |
| APC‐H7 | CD8* | SK1 | CD45RO* | UCHL1 | CD20* | L27 | CD3*, CD19*, CD20* | SK7, HIB19, L27 |
| V450 | CD3* | UCHT1 | CD3* | UCHT1 | CD14* | MoP9 | ||
| BV421 | CD27** | O323 | ||||||
| V500 | HLA‐DR* | L243(G46‐6) | HLA‐DR* | L243(G46‐6) | CD3* | UCHT1 | HLA‐DR* | L243(G46‐6) |
| BV605 | CD196 (CCR6)** | G034E3 | ||||||
Reagent manufacturer:
*
Becton Dickinson (BD);
**
BioLegend;
***
Beckman Coulter;
****
Miltenyi Biotec. FITC = fluorescein isothiocyanate; PerCp = peridinin chlorophyll; PE‐Cy7 = phycoerythrin‐cyanin 7; HLA‐DR = human leukocyte antigen DR. APC = allophycocyanin.