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. Author manuscript; available in PMC: 2020 Sep 15.
Published in final edited form as: Life Sci. 2019 Jul 26;233:116699. doi: 10.1016/j.lfs.2019.116699

Figure 7: Denervation upregulates UCHL1 in skeletal muscles, but ablation of UCHL1 in skeletal muscles fails to prevent denervation-induced atrophy.

Figure 7:

(A) Representative images of Western blots for UCHL1 and GAPDH in soleus and EDL from WT and skmKO mice after 14 days of denervation. (B) Quantifications of Western blots for UCHL1 in soleus and EDL from WT (n=4) and skmKO (n=5). Results were normalized to WT innervated soleus, black bars are samples from innervated muscles, and grey bars are data from denervated muscles, “*” indicates p < 0.05 as compared between WT innervated vs WT denervated group (denervation effect), by two-way ANOVA followed by t-test. (C) Immunohistochemistry staining of cross sections of innervated and denervated gastrocnemius from WT (n=4) and UCHL1 skmKO (n=3) mice, stained with Dystrophin (Red). The scale bars indicate 20 μm. (D) Measurement of denervated gastrocnemius muscle fiber cross-section area of 150 fibers in three areas from WT mice (n=4) and skmKO mice (n=3).