Fig. 5.

Localization of mtor transcripts in the testis and pituitary of Astatotilapia burtoni labeled by chromogenic in situ hybridization. (A) Transverse sections through the testis shows positive staining with anti-sense mtor probe (purple label) (A), and absence of staining with sense control probe (B). (C) Higher magnification of the testis shows mtor expression (anti-sense probe) within membrane-bound spermatocysts containing germ cells of all spermatogenic stages, but no expression in mature sperm (spm) located in the center of the tubules or in interstitial tissue (it). (D) Alternate section of that shown in C stained with sense control probe shows no staining. (E) Transverse sections through the pituitary and hypothalamus shows positive mtor expression throughout the proximal pars distalis (PPD), with concentration at the border between the pars nervosa (PN) and PPD (arrows). Positive staining is also observed in the ventral (NLTv) and intermediate (NLTi) parts of the lateral tuberal nucleus. The sense probe shows no staining (F). AS, anti-sense probe; bv, blood vessel; S, sense probe. Scale bars=250 μm (A,B); 20 μm (C,D); 100 μm (E,F).