Fig. 6.

Expression of mtor mRNA levels in the pituitary changes during social ascent in male Astatotilapia burtoni. (A) Relative mtor mRNA levels do not differ during the first 24 hrs of social ascent, but are higher at 72 hrs and 120 hrs. Levels also do not differ between subordinate and dominant males. Box boundaries show 25^th and 75^th percentiles, whiskers represent 10^th and 90^th percentiles, solid line is the median, and dotted line is the mean. Dots represent points outside the 10^th and 90^th percentiles. Different letters indicate statistical differences at p<0.05 and sample sizes are shown in parentheses. (B) Relative mtor mRNA levels in the pituitary are positively correlated with lhβ mRNA levels, but not fshβ levels. qPCR data were normalized to the geometric mean of the reference genes, 18s and gapdh.