Fig. 7.
Expression of mtor mRNA levels in the testes changes during social ascent in male Astatotilapia burtoni, and is positively correlated with follicle stimulating hormone receptor. (A) Relative mtor mRNA levels in the testes do not differ during the first 72 hrs of social ascent, but are higher at 120 hrs. Levels are also higher in dominant compared to subordinate males. Box boundaries show 25th and 75th percentiles, whiskers represent 10th and 90th percentiles, solid line is the median, and dotted line is the mean. Dots represent points outside the 10th and 90th percentiles. Different letters indicate statistical differences at p<0.05 and sample sizes are shown in parentheses. (B) mtor mRNA levels are positively correlated with fshr mRNA levels in the testes. Each point represents the mean values of all animals within that social time point. qPCR data were normalized to the geometric mean of the reference genes, 18s and gapdh.
