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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Proteins. 2019 Jun 12;87(10):878–884. doi: 10.1002/prot.25751

Figure 1. Rational design of μ-OR stabilizing and destabilizing mutants.

Figure 1.

(A) Eris scan identifies a stabilizing mutation (T315Y) and destabilizing mutation (R165Y) using μ-OR structural models (Left: inactive state, Right: active state). The mutated tyrosine residues were shown as spheres.

(B) T315Y can mediate hydrogen bond network (Y128-H319-T315Y) and aromatic interactions (T315Y-W318) in the design model, while R165Y disrupts polar interactions with D124 and T279 of μ-OR.