Fig. 5.

The blue (a), the green (b), and the orange (c) histograms show the inhibitory effect of Zn-Porph (added to monomeric Aβ40 in DMSO in a 1:1 M ratio) on the formation of soluble monomeric and dimeric species of Aβ40 selected as dt_m/z marker pairs for the sample sets at 5 μM (a), 20 μM (b), and 20 μM incubated before injection (c). The height of each bar represents the reduction (in %) of the average ion intensity of each dt_m/z marker pair measured in the “Aβ40 plus Porph” sample class with respect to the average intensity measured in the “Aβ40” class. The species M⁴⁺(6.28_1082.79), M³⁺(7.83_1443.39), D⁵⁺compact (7.28_1731.87) conformation, D⁵⁺extended (10.80_1731.87) conformation, and D⁴⁺(10.36_2164.58) were all selected in the three sample sets as dt_m/z marker pairs to evaluate the inhibitory effect of Zn-Porph on their formation. In the sample set at 5 μM (a), the feature M²⁺(16.10_2164.58) was instead excluded due to its high intra-class intensity variability (p(corr)[1] < + 0.9)