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. 2019 Sep 2;10:3945. doi: 10.1038/s41467-019-11928-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Altered DA neuron electrophysiological properties in 4-month-old Syn rats. a Spontaneous firing in SNpc DA neurons from 4-month-old rats (scale: 2 min, 0.2 mV) and plots showing firing frequency (38 cells from 10 WT, 47 cells from 11 Syn; Mann–Whitney test, ***P < 1.00 × 10⁻⁴) and coefficient of variation of interspike interval (35 cells from 10 WT, 45 cells from 11 Syn; Mann–Whitney test, **P = 0.002). b APs induced by depolarization (scale: 100 ms; 20 mV, 100 pA) from DA neurons held at −50 mV and mean (±s.e.m.) AP numbers (11 WT, 10 Syn neurons, 3 rats each; two-way repeated-measures ANOVA: genotype×current, F_4,76 = 9.36, P < 1.00 × 10⁻⁴; current, F_4,76 = 116.6, P < 1.00 × 10⁻⁴; genotype, F_1,19 = 10.76, P = 0.004. WT vs Syn: 200 pA ***P < 1.00 × 10⁻⁴, 150 pA ***P = 4.00 × 10⁻⁴, 100 pA *P = 0.036, 50 pA P = 0.221 with Bonferroni’s). c Sub-threshold responses to hyperpolarization (scale: 100 ms; 10 mV, 100 pA) and current/voltage plots (±s.e.m.; 11 WT, 10 Syn neurons, 3 rats each; two-way repeated-measures ANOVA: genotype×current, F_4,76 = 1.64, P = 0.172; current, F_4,76 = 362.9, P < 1.00 × 10⁻⁴; genotype, F_1,19 = 1.26, P = 0.276. WT vs Syn: 0 pA P > 0.999, −50 pA, P > 0.999, −100 pA, P > 0.999, −150 pA, P = 0.496, −200 pA, P = 0.411 with Bonferroni’s). d Cm (23 WT, 29 Syn neurons, 5 rats each: Welch’s t-test P = 0.638). e I_h currents from DA neurons held at −60 mV and hyperpolarized in 20 mV increments (scale: 200 ms; 50 mV, 200 pA). The activation phase was fitted to 1–2 exponentials (dash line). The plots show I_h amplitude and weighted activation tau after hyperpolarization to −120 mV (24 WT, 22 Syn neurons, 8 rats each; amplitude: Welch’s t-test P = 0.886; tau: Mann–Whitney test P = 0.342). f AHC (scale: 100 ms; 50 mV, 50 pA), induced by depolarization to 0 mV. AHC trace area is designated by red dash lines and deactivation is obtained with exponential fits (12 WT, 21 Syn neurons, 4 rats each; area: Welch’s t-test ***P = 3.00 × 10⁻⁴; tau: Mann–Whitney test *P = 0.013). Source data are provided as a Source Data file