Fig. 5.

CD8 T cell priming, expansion, and generation of long-lived memory precursors is not enhanced in LARC GAP immunized IFNAR^−/− mice. a Schematic of the immunization regimen. B6 mice and IFNAR^−/− mice were immunized once with 50,000 LARC GAP or LARC GAP OVA sporozoites and liver lymphocyte populations analyzed either at day 7 or day 14 post immunization. b Analysis of the proportion (left panel) and total number (middle) of OVA-specific and bulk activated (right panel) liver CD8 T cells, 7 days after immunization. OVA-specific CD8 T cells are characterized as OVA+ of CD44hiCD62Llo CD8+CD4−CD3+ T lymphocytes. Bulk activated CD8 T cells are characterized as CD44^hiCD62L^lo CD8+CD4−CD3+ T lymphocytes. c Analysis of short-lived effectors (SLEC) and long-lived memory precursor effector cells (MPEC) at day 14 post primary immunization. Gating strategy showing KLRG1^hiCD127^lo SLEC and KLRG1^loCD127^hi MPEC CD8 T cells in the livers of immunized mice (top panel). Quantification of the total number of liver leukocytes (bottom, left panel), proportion of MPECs (bottom, middle panel), and total number of MPECs (bottom, right panel). Data are compiled from two independent experiments with two mock immunized and three LARC GAP immunized mice in each group. Each dot represents an individual mouse. Bar graphs are expressed as mean ± SD. *p < 0.05, and **p < 0.005 (from unpaired two-tailed Student’s t-test). Components of this figure were created using Servier Medical Art templates, which are licensed under a Creative Commons Attribution 3.0 Unported License; https://smart.servier.com