Fig. 6.

The detrimental role of the parasite-engendered type I IFN response is extrinsic to the CD8 T cell compartment. a Schematic of OT-1 adoptive transfer and subsequent immunization regimen. WT B6 mice and IFNAR^−/− mice received 100,000 naïve OT-1 cells 24 h prior to immunization with 50,000 LARC GAP OVA sporozoites. Thirty days later, liver non-parenchymal cells were isolated and subjected to FACS analysis. b Quantification of OT-1 CD8 T cells by FACS analysis. OT-1 T cells were identified using the SIINFEKL MHC-I restricted OVA tetramer. c Analysis of OT-1 liver memory CD8 T cells 30 days after GAP immunization. liver memory CD8 T cells are defined as CD69+CD62L^lo of CD44^hiOVA+CD8+CD4−CD3+T lymphocytes. d Analysis of PD-1 expression on OT-1 memory CD8 T cells. PD-1+ cells are defined as PD-1+ of CD44^hiOVA+CD8+CD4−CD3+ T lymphocytes. Data from panels b through d are compiled from two independent experiments with two mock immunized and three GAP immunized mice in each group. Each dot represents a single mouse. Bar graphs are expressed as mean ± SD. *p < 0.05 (from unpaired two-tailed Student’s t-test). Components of this figure were created using Servier Medical Art templates, which are licensed under a Creative Commons Attribution 3.0 Unported License; https://smart.servier.com