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. 2019 Sep 3;5:132. doi: 10.1038/s41420-019-0212-4

Fig. 2. PERP protein is stabilized by ER stress, independent of p53 transcriptional regulation.

Fig. 2

HCT116 cells were treated with 1 μg/ml BFA for the indicated time points and changes to the levels of a SERCA2b mRNA (n = 4, one-way ANOVA, F = 3.442, p = 0.0239*), b SERCA2b protein (n = 7), c PERP protein (n = 7, one-way ANOVA, F = 3.025, p = 0.0329*) and d PERP mRNA (n = 4, one-way ANOVA, F = 5.297, p = 0.0036**) were detected by RT-PCR or Western blot and normalized to the level of GAPDH. e HCT116 p53−/− cells were treated with 1 µg/ml BFA and the response of PERP protein levels was detected by immunoblotting