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. 2019 Sep 2;10:3925. doi: 10.1038/s41467-019-11760-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — PARP mediates repair of replication stress-associated DSBs and MSI induction in Msh2^−/− MEFs. a, b Msh2^+/+ and Msh2^−/− MEFs were treated according to the workflow (upper box). γH2AX foci (a n numbers are indicated in graph) and γH2AX/53BP1 foci (b) were detected by immunofluorescence. Percentages of γH2AX foci merged with 53BP1 foci (means ± s.e.) are indicated in each image (b). Bars show means ± s.d. Scale bars, 10 μm. Two-tailed Welch’s t-test was used for statistical analysis. c Msh2^−/− MEFs were cultivated under the Std-3T3 protocol and continuously treated with Olaparib, and their CIN-induction and MSI statuses were monitored. The MSI status was determined at the indicated loci. The CIN-induction status was determined by flow cytometry. Green and red bars indicate diploidy and tetraploidy, respectively