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. 2019 Sep 2;9:12642. doi: 10.1038/s41598-019-49100-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Establishment of ANTXR1-downregulated or integrinβ1-downregulated BMSC sheets. (a) Fluorescent microscopy images of BMSC sheets transfected with ANTXR1-shRNA at MOI = 40. Scale bar = 500 µm. (b) RT-PCR was performed to determine the mRNA expression levels of ANTXR1. (c,d) Western blotting was performed to determine the protein expression levels of ANTXR1, followed by the quantitative analysis of the western blotting bands by ImageJ software. (e) Fluorescent microscopy images of BMSC sheets transfected with integrinβ1-shRNA at MOI = 40. Scale bar = 500 µm. (f) RT-PCR was performed to determine the mRNA expression levels of integrinβ1. (g–h) Western blotting was performed to determine protein expression levels of integrinβ1, followed by the quantitative analysis of the western blotting bands by ImageJ software.