Table 2.
Limiting dilution xenotransplantation summary representing tumor CSC frequency during propagation
| Number of cells injected | Engraftment rate | Stem cell frequency* | |
|---|---|---|---|
| Early | 1000 | 3/4 | 1/1581 |
| 500 | 0/4 | ||
| 100 | 0/4 | ||
| Late | 1000 | 3/4 | 1/304 |
| 500 | 4/4 | ||
| 100 | 2/4 | ||
| 50 | /14 | ||
| 10 | 0/4 |
Summary of CSC frequency estimated by limiting dilution xenotransplantation of cells isolated from early and late PDX passages. The data is presented as the ratio of injections that formed tumors within 12 weeks. Bold numbers represent the estimated CSC frequency. In the comparison between early and late PDX passages. Estimation of the relative frequency of cancer propagating cells was calculated online using the ELDA software online (http://bioinf.wehi.edu.au/software/elda/)
*p = 0.009