Fig. 6.

Incorporation of ncAA p-azido-l-phenylalanine (pAzF) by orthogonal Ribo-T. a Combined rRNA and sf-gfp plasmid with sf-gfp gene is replaced with a 1TAG or 5TAG version to create pORT3B.gfp1TAG and pORT3B.gfp5TAG (orthogonal Ribo-T with ASD sequence 3 and orthogonal sfGFP message B containing 1 TAG or 5 TAG, respectively). Wild-type rrnb operon was cloned as a negative control for background orthogonal expression (pAM.B.gfp1TAG and pAM.B.gfp5TAG). b Expression of sf-gfp with 1TAG or 5TAG in C321.ΔA derived strain MCJ.1217 (C321.ΔA.mutS⁺.Δλred.Δupp), in the presence of (+) or absence of (−) pAzF. Error bars = 1SD of n = 6 independent experiments. Source data for b can be found in the Source Data file