Fig. 3.

Palmitoylation of 5-HT1AR and expression of ZDHHC21 are decreased in the forebrains of mice with depressive-like syndrome. a Schematic diagram of experimental design for mouse model of depression. b Results of the sucrose preference test performed in control (n = 7) and chronically stressed mice (n = 19). c Immobility time in the forced swim test of control and chronically stressed mice. Group of stressed mice was then divided into anhedonic (anhed) (n = 12) and resilient (res) (n = 7) animals and immobility time was analyzed in each subgroup. One-way ANOVA. Data points represent mean ± SEM (*P < 0.05). d In anhedonic and resilient mice, palmitoylation of the 5-HT1AR was analyzed in the prefrontal cortex (PFC) and cerebellum by acyl-biotinyl exchange (see also Supplementary Fig. 5A–E). e Quantification of 5-HT1AR palmitoylation in anhedonic and resilient animals (see also Supplementary Fig. 5A–E). f Relative expression levels of ZDHHC5, -9, and -21 in the PFC of resilient and anhedonic mice assessed by quantitative reverse transcriptase-PCR. *P < 0.05; two-tailed t test. (See also Supplementary Fig. 5F–H). Source data are available as a Source Data file