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. 2019 Sep 2;9:12658. doi: 10.1038/s41598-019-49038-8

Figure 4.

Figure 4

Deficiency of A20 results in impaired T cell development. (A–D) FACS plots (A,C), frequencies and absolute numbers (B,D) exhibiting differentiation of T cells from the thymus of 14 days old A20Hem-KO and control mice (n = 5). (A,B) Double negative cells to single positive T cells; (C,D) DN1 to DN4 cells. (E) Frequencies of T cells from the spleen of 14 days old A20Hem-KO and control mice (n = 5). (F) FACS plots and frequencies of CD4+ T cells and CD8+ T cells from the spleen of 14 days old A20Hem-KO and control mice (n = 3). (G,H) FACS plots (G), frequencies and absolute numbers (H) of naïve, effector and memory T cells from the spleen of 14 days old A20Hem-KO and control mice (n = 3). (I) FACS plots and frequencies of T cells from the peripheral blood (PB) of 14 days old A20Hem-KO and control mice (n = 2). All data represent mean ± SEM. Two-tailed student’s t tests were used to assess statistical significance (*P < 0.05, **P < 0.01, ***P < 0.001).