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. 2019 Jul 30;60(9):1590–1602. doi: 10.1194/jlr.RA119000251

Fig. 3.

Fig. 3.

Dnr and Ara-C selection pressure elicits ceramide resistance in HL-60 cells. A: Sensitivity to C6-ceramide. Cells (50,000/0.2 ml media per well/96-well plate) were exposed to C6-ceramide at the concentrations shown for 72 h. B: Sensitivity to C18-ceramide. HL-60 wt and HL-60/dnr cells (300,000/1.2 ml media per well/24-sell plate) were exposed to C18:0 (5.0 μM) ceramide for 24 and 48 h. Experiments were conducted in dnr- and Ara-C-free media. Viability was measured by PI staining (A) and assessed by automated cell counting using trypan blue (B). C6-ceramide was delivered in DMSO. DMSO at all concentrations had no effect on cell viability. Controls contained vehicle. C18:0 ceramide was delivered to cells in ethanol vehicle (0.1% final concentration) as detailed in Materials and Methods. Ethanol had no effect on cell viability. *Statistical significance versus 24 h treatment HL-60/dnr cells; **statistical significance versus 24 h treatment wt HL-60 cells; #statistical significance (p <0.05) versus wt HL-60 cells at 48 h.