Fig. 7.
Temperature dependence of the lipid visibility Irel of specific functional groups. Some well-separated lipid NMR signals were evaluated. To improve accuracy, the baseline around each signal was adjusted separately, and the integral limits were framed into local minima next to the peaks. Note that the temperature dependence of the methyl signal (A) represents the FAs only since the cholesterol signal was first subtracted as described before (see also Fig. 6). The overlaying methanol signal next to the choline head group [C, -N(CH3)3, ∼3.2 ppm] in extracts was subtracted by deconvoluting the spectra, as was discussed earlier. The resulting integrals were compared with the corresponding integrals of the extracted lipids, respectively. The temperature dependency of Irel was fitted with equation 8; the fit parameters are listed in Table 4.